Postdoctoral Fellow

University of South Alabama Mitchell Cancer Institute

Mobile, AL

Job posting number: #7058353

Posted: July 10, 2019

Job Description

JOB PURPOSE: This multidisciplinary work seeks to validate novel mechanisms linking B-vitamin cofactors to cellular dysfunction and onset of disease upon xenobiotic exposure. This cell-based work encompasses the development of biological methods that interrogate the effects of xenobiotics and endogenously generated toxins on the metabolic pathways of B-vitamins derived cofactors and probe their impact on cellular events, ranging from the changes to central metabolism and post-translational modifications, to DNA damage and repair and regulation of gene expression. Vitamin B modulations are to be achieved through supplementation, environmental or chemotherapeutic exposure, or combinations of the three. This work is directly embedded in the MCI Clinical Cancer Research activities.

The Gassman lab has extensive expertise in DNA damage response and repair and have developed novel assays for examine damage induction and repair. In collaboration with the Migaud group, the researcher will be trained in these methods to provide mechanistic insight into the roles vitamin B1, B2 or B3 derived cofactors play in cellular dysfunction, mitochondrial dysfunction, and DNA repair. In addition to contributing to the group’s research effort, the successful applicant will be expected to contribute to the day to day running of the Migaud laboratory and closely interact with the Gassman group and his/her biologist and chemist colleagues working on this project.

1. Utilization of well-established cell based assays to characterize the cytotoxicity and genotoxicity of B-vitamin cofactors. These assay include, cell viability, detection of reactive oxygen species and oxidative stress, DNA adduct detection and strand breaks via gamma-H2AX signaling, cell cycle alterations, and mitochondrial dysfunction.
2. Work as part of a team of researchers composed of trainee students, PhD and experienced researchers working at the interface between chemical, biochemical and biological sciences.
3. Make periodic presentations and contribute actively to the overall research group report activities.
4. Contribute to the preparation of Migaud/Gassman grant funding applications and apply for individual fellowships, awards, project or travel support.
5. Contribute to the generation of high impact publications under the supervision of Dr Migaud and in collaboration with the Gassman laboratory.
6. Attend and present their findings at research group meetings and at national or international meetings as required.
7. Assist in the supervision of post-graduate students or project students
8. To contribute to the maintenance of pieces of bioanalytical equipment, as well as that of general biology laboratory equipment and facilities
9. Read academic papers, journals and textbooks to keep abreast of developments in own specialism and related disciplines
10. Carry out routine administrative tasks associated with the research project/s to ensure that the projects milestones are completed on time and within budget. These tasks will include financial control and risk assessment of research activities.

• Independent, day to day planning of experiments and 1-2 month (short-term) planning of research.
• 4-6 months (long-term) organization of the research direction/targets as well as contingency planning.
• Prioritize and reprioritization of research/experiments in order to meet deadlines and targets.
• Organize informal meetings communicate directly with other lab members or with supervisor.

• Support the development and training of other staff in the group by making available their research experience and expertise. Contribute to the mentoring of undergraduate and graduate students.
• Take shared responsibility for the upkeep of lab equipment and replenishment of lab stocks and exercise due diligence when using equipment.

• Keep accurate records of experimental designs and assist in the development of robust protocols for distribution to collaborators.
• Communicate openly with colleagues’ latest research findings and results. Assist in troubleshooting and knowledge development amongst the group.
• Develop contacts with other labs within the research community at USA and MCI and look to identify potential cross-discipline collaborations.

Employment/Probationary Period
• The position is for 2 years, renewable after 1 year following adequate progress.
• The position comes with a 6-month probationary period.
• The review process will be conducted by Dr Migaud and confirmed by the collaborating team (Gassman) following recommendation by Dr. Gassman/Dr. Migaud

Have a PhD in Biochemistry, Biology or equivalent.. A BSc degree (equivalent or above) in Chemistry, Biochemistry,
Biology or equivalent.

Expertise in cell biology, in particular techniques used to explore cell survival mechanisms, oxidative stress, DNA
damage/repair pathways, cell cycle alterations, and mitochondrial dysfunction.
Experience in cellular metabolic dysfunction.
Experience with xenobiotic exposure.
Recent experience in exploring metabolic pathways to investigate cellular mechanisms' dysfunction
Publication record commensurate with stage of career
Recent experience in cell biology

Robust understanding of the practical application of biological principles (eg. experimental design, blots, cell-based procedures, cell line characterization, etc).
Robust bioanalytical skills commensurate with biological expertise.
Appreciation of chemical biology and understanding of small molecules' role in controlling biological events
Prior work on B-vitamin related cell biology

Excellent communication skills.
Evidence of presentation at national or international conferences (poster and/or oral).

Must demonstrate good team working skills.
Must demonstrate a true commitment to and interest in research.
Must be willing to work irregular hours when necessary for the progress of the research project.
Must be willing and able to travel to national and international meetings if required
Experience of participation in training/mentoring or students or junior staff

Positions will be within the Department of Oncologic Sciences and the Mitchell Cancer Institute at the University of South Alabama. To apply, please send your curriculum vitae to:

Applications will be reviewed and evaluated on an ongoing basis.
The University of South Alabama is an Equal Opportunity Employer
Minorities/Females/Veterans/ Disabled.

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