Senior Associate Scientist
South San Francisco, CA
Job posting number: #7121829 (Ref:4876201 )
Posted: January 23, 2023
Application Deadline: February 10, 2023
Senior Associate Scientist, High-Throughput Protein Production
Pfizer’s BioMedicine Design (BMD) High-Throughput Protein Production (HTP) group is seeking a highly motivated scientist to manage and perform antibody expression and purification, from hundreds to thousands of proteins per week. The candidate will play a critical role in production and automation process innovation in a core group that is responsible for high-throughput cloning, expression & purification of antibodies (microgram-to-milligram scale).
The candidate will be part of a team responsible for high throughput automated transfection, purification, quantitation, analytical assessment, and normalization/delivery of protein samples, including conventional antibodies, bispecifics, and occasional non-antibody proteins; data upload in the departmental database/ELNs; and interacting with project teams. The candidate will contribute to group-wide deliverables, work on several projects simultaneously, explore new purification technologies, and present results and updates at group, project, and departmental meetings.
How You Will Achieve It
High-throughput automated antibody purification, quantitation, analytical SEC, normalization, and redox for bispecific antibody generation utilizing the team’s liquid handling automation equipment.
Optimization of automated processes throughout the HTP workflow
Maintaining mammalian cell stocks, transfecting and harvesting conditioned medium in small- and micro-scale.
Managing the purification database, scheduling purifications, and delivering custom arrayed materials to downstream users
Maintaining & troubleshooting of liquid handlers
Development of automated bispecific antibody generation methods using different antibody formats
Close interaction with protein engineering project teams to plan and coordinate protein production
Presentation of purification data at group & project team meetings
Regular review of literature to maintain knowledge of scientific trends and industry processes, advance molecular biology and protein production methods
Bachelor’s degree & 2-4 years of experience, or master’s degree and 1-2 years of experience, in protein purification, biochemistry, biology, chemical engineering or a related discipline, along with a basic understanding of antibodies and protein production.
Demonstrated technical proficiency in protein purification
At least 2 years of experience with using laboratory automation, including writing and troubleshooting protocols
Track record of strong problem solving and organizational skills, orientation to detail
Ability to multi-task, prioritize responsibilities, work under strict deadlines, and remain flexible with changing needs
Ability to work effectively to solve process development problems and implement improved procedures.
Strong written and oral communication skills
Experience with Tecan, Beckman, and/or Lynx liquid handling platforms
Industry experience in protein expression/purification/antibodies/ molecular biology
Experience with high throughput sample tracking and laboratory logistics software
Experience in writing and understanding methods for laboratory automation technologies.
Lifting, sitting, standing, walking, bending, reaching, ability to perform mathematical calculations and ability to perform complex data analysis.
Other Job Details:
Last Day of application: February 9th, 2023
Work Location Assignment: On Premise, colleagues work in a Pfizer site because it’s needed to get their job done. They may have flexibility to work remotely from time to time, but they are primarily on-site.
Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.